At four different steps prior to
microarray hybridization quality
controls are performed:
1) The integrity of total RNA is examined on an Agilent
2100 Bioanalyzer. This is basically a miniaturized electrophoresis
device for nucleic acids; results are depicted as an
electropherogram or in a "gel view mode". For examples of "perfect" RNAs and RNAs
degraded to varying degrees see
or download this file: ppt (345kB).
2) cDNA yield is determined photometrically (A260).
3) cRNA yield and the efficiency of Cy3- or Cy5-incorporation
are determined photometrically (A550 or A650 /A260).
4) The distribution of cRNA fragments is analyzed on Agilent
2100 Bioanalyzer. For examples see
or download this file: ppt (110kB).