History of the microarray project


Januar 2001

Start of the new SFB566 research initiative.

Summer 2001

Selection of an initial gene list based on a literature search.

Autumn 2001

Final decision (HBFG procedure) to reward a grant of 225.000,- of investments, a post doc and a technician to the microarray project.

Autumn 2001

Design of three oligonucleotides per gene by algorithms developed at MWG Biotech, synthesis of oligonucleotide probes, printing of the first version of the inflammation array at MWG Biotech.

December 2001

The first microarray experiment was performed.

Spring 2003

Final validation of the human and murine probes was performed in 214 and 87 hybridizations, respectively. The underlying material for the validation experiments was derived from the participating projects of the SFB566 and external collaborators. Altogether more than 25 different cell types (view list) and more than 30 different stimuli (view list) were used.

June 2003

Final selection of probes and printing of the 2nd generation Inflammation array. This microarry is commercialised by MWG Biotech.

May 2003

The Medical School Hannover grants a full position for a bioinformatician to the project.

June 2003

The SFB566 and the microarray project are reviewed by the DFG and achieve together a further 4 year funding of 8 million .

December 2004

MWG Biotech stops producing microarrays and stops selling the Inflammation microarrays.

June 2005

The contract with MWG Biotech has been terminated. The Inflammation arrays are now sold by Ocimum.

July 2005

CytoBASE is implemented and accessible via a WEB interface, more than 1000 microarray experiments have been performed.

July 2006

The high-throughput Agilent Microarray Scanner G2565BA is purchased. It enables scanning of up to 48 slides within a single run.

January 2008

The last 4-years funding period of the SFB566 and the microarray project starts.

September 2009

The Agilent Microarray Scanner is upgraded to Version G2565CA resulting in a maximum resolution of 2m and allowing to scan slides that carry up to 1 Million oligonucleotide probes.

January 2010 A signal transduction map of IL-1 is published.
Weber A, Wasiliew P, Kracht M. Interleukin-1 (IL-1) processing pathway. Sci Signal. 2010 Jan 19;3(105):cm1 PubMed link to full text
February 2010 A total number of 3000 Microarray hybridizations has been achieved.
September 2010 A total number of 30 publications involving the microarray lab have been published (for an updated list see publications).

December 2010

The cooperation with Ocimum is terminated. Ocimum stops selling the Inflammation microarrays.

July 2011 A boolean model of the IL-1 network is published.
Schmitz ML, Weber A, Roxlau T, Gaestel M, Kracht M. Signal integration, crosstalk mechanisms and networks in the function of inflammatory cytokines. Biochim Biophys Acta. 2011 Dec;1813(12):2165-75. Epub 2011 Jul 20 PubMed
August 2011 Proposals for 2 complementary research initiatives (CRC 967 and TRR116) each involving the microarray lab in Hannover as central service project providing transcriptomics technologies are completed and sent to the DFG.
December 2011 The last funding period of SFB566 ends.
January 2012 By the end of the SFB566 the microarray lab in Hannover continues to work as a transcriptomics core facility of the Medical School Hannover.